ModellingandOptimizationofthe(R)-(+)-3,4-dihydroxyphenyllactic AcidProductionCatalyzedwithD-lactateDehydrogenase fromLactobacillusleishmanniiUsingGeneticAlgorithm
نویسنده
چکیده
A mathematical model for the enzymatic kinetics of the synthesis of (R)–(+)–3,4-dihydroxyphenyllactic acid (DHPL) was developed. The synthesis was catalyzed by D-lactate dehydrogenase from Lactobacillus leishmannii. Since this enzyme requires NADH as a coenzyme, formate dehydrogenase system was used for NADH regeneration. Kinetic constants of both enzymes were estimated independently from initial reaction rate experiments. The developed mathematical model was verified by the batch reactor experiment (volumetric productivity in this experiment was 4.76 g dm–3 d–1). Optimization of initial reaction conditions for DHPL synthesis was performed using the genetic algorithm (GA). The genetic algorithm as a flexible optimization tool had been used to obtain the experimental conditions where maximal volumetric productivity could be achieved. The optimal initial conditions were found in the investigated parameter area: c3,4-dihydroxyphenylpyruvic acid = 4.69 mmol dm–3, cNAD+= 4.95 mmol dm–3, cformate = 36.85 mmol dm–3, D-lactate dehydrogenase = 3 mg cm–3, formate dehydrogenase = 2.94 mg cm–3 and the reaction time 8.5 min. At these conditions volumetric productivity of 93.06 g dm–3 d–1 can be achieved.
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تاریخ انتشار 2004